Journal: Oncogene

Article Title: AGR2 is a SMAD4-suppressible gene that modulates MUC1 levels and promotes the initiation and progression of pancreatic intraepithelial neoplasia

doi: 10.1038/onc.2012.394

Figure Lengend Snippet: (A) Quantitative RT-PCR of AGR2 mRNA, 24 hrs after addition of 500 pM TGF-β1, in ASPC-1, BxPC3, COLO-357, and PANC-1 cells. Data are the means ± SEM from at least three experiments. * p < 0.01, compared with respective controls. (B) The levels of AGR2 RNA were determined by quantitative RT-PCR following addition of 500 pM TGF-β1 for 0, 1, 3, 8, 12, 16, and 24 hrs in COLO-357 (white) and PANC-1 (black). The points plotted are the average of two experiments at each time point. (C) Western blot of AGR2, SMAD4, and ERK2 (loading control) in ASPC-1, BxPC3, COLO-357, and PANC-1 cells after 48 hrs of incubation with 500 pM TGF-β1. T3M4 cells had no detectable levels of AGR2 protein. (D) Densitometry of AGR2 immunoreactivity following 48 hrs of TGF-β in ASPC-1, BxPC3, COLO-357, and PANC-1. The mean pixel density of AGR2 was quantitated and normalized to its corresponding ERK2 (loading control). Data are the means ± SEM from at least three experiments.* p < 0.01, compared to untreated control. (E) Western blot of AGR2 and ERK2 (loading control) in PANC-1 cells after 16, 24, and 48 hrs incubation with TGF-β1. (F) Western blot of AGR2 and ERK2 (loading control) in COLO-357 after 16, 24, and 48 hrs incubation with 500 pM TGF-β1.

Article Snippet: Plasmids were obtained from: SBE4-Luc TGF-β reporter (Addgene #16495; [ ]), pCMV5-DPC-HA SMAD4 (Addgene #14038; [ ]), two pTRIPZ-AGR2 plasmids (Open Biosystems, Huntsville, AL; V2THS_251763, V2THS_199455), pcDNA-AGR2 and AGR2-RFP (Ted R. Hupp; University of Edinburgh).

Techniques: Quantitative RT-PCR, Western Blot, Control, Incubation

Journal: Oncogene

Article Title: AGR2 is a SMAD4-suppressible gene that modulates MUC1 levels and promotes the initiation and progression of pancreatic intraepithelial neoplasia

doi: 10.1038/onc.2012.394

Figure Lengend Snippet: (A) Quantitative RT-PCR of AGR2 RNA levels in ASPC-1, BxPC3, COLO-357, and PANC-1 cells with CMV-HA sham alone, CMV-SMAD4, CMV-HA sham/TGF-β, or CMV-SMAD4/TGF-β. Data are the means ± SEM from at least three experiments. * p < 0.05, and ** p < 0.01 compared to respective controls. (B) A western blot showing SMAD4, AGR2, and ERK2 (loading control) protein levels in COLO-357 cells stably expressing either pGIPZ-SMAD4 (left) or pGIPZ-scrambled (right) shRNA silencing vectors, with or without treatment with TGF-β. (C) A luciferase assay using the AGR2-luc reporter with either wild-type, mutated SBE1, mutated SBE2, or with both SBEs mutated and with co-transfection of either CMV-HA sham or CMV-SMAD4 in PANC-1. Percent luciferase units relative to wild-type and untreated AGR2-luc control are shown, after normalization to a Renilla internal control for transfection and cell lysis. Data are the means ± SEM from three experiments. * p < 0.01 compared with respective control.

Article Snippet: Plasmids were obtained from: SBE4-Luc TGF-β reporter (Addgene #16495; [ ]), pCMV5-DPC-HA SMAD4 (Addgene #14038; [ ]), two pTRIPZ-AGR2 plasmids (Open Biosystems, Huntsville, AL; V2THS_251763, V2THS_199455), pcDNA-AGR2 and AGR2-RFP (Ted R. Hupp; University of Edinburgh).

Techniques: Quantitative RT-PCR, Western Blot, Control, Stable Transfection, Expressing, shRNA, Luciferase, Cotransfection, Transfection, Lysis

Journal: Oncogene

Article Title: AGR2 is a SMAD4-suppressible gene that modulates MUC1 levels and promotes the initiation and progression of pancreatic intraepithelial neoplasia

doi: 10.1038/onc.2012.394

Figure Lengend Snippet: (A) Co-immunofluorescence in a Pdx1-Cre/Kras G12D /p53 L/L model. Shown are mouse low-grade PanIN lesions stained for AGR2 (red), MUC1 (green), and DAPI (blue). The merged image from AGR2 and MUC1 is shown in the far right panel. Yellow color indicates areas of co-localization of AGR2 and MUC1. Magnification: 100×; Scale bar: 40 μm. (B) Alcian blue staining combined with immunohistochemical detection of AGR2 in mPanIN-3 and early invasive adenocarcinoma from a Pdx1-Cre/Kras G12D /Smad4 L/L model. Magnification: 200×; Scale bar: 20 μm. (C) Immunohistochemistry of AGR2 (left) and MUC1 (right) in serial sections of human pancreas, with the same area of the mPanIN structure magnified in an enlarged view. An mPanIN-2 lesion is seen on the left, within a larger area of ADM. Early adenocarcinoma shown on the right, and is enlarged in the box. Invasive clusters surround the larger lesion and stain highly for AGR2. Magnification: 100×; Scale bar: 40 μm.

Article Snippet: Plasmids were obtained from: SBE4-Luc TGF-β reporter (Addgene #16495; [ ]), pCMV5-DPC-HA SMAD4 (Addgene #14038; [ ]), two pTRIPZ-AGR2 plasmids (Open Biosystems, Huntsville, AL; V2THS_251763, V2THS_199455), pcDNA-AGR2 and AGR2-RFP (Ted R. Hupp; University of Edinburgh).

Techniques: Immunofluorescence, Staining, Immunohistochemical staining, Immunohistochemistry

Journal: Oncogene

Article Title: AGR2 is a SMAD4-suppressible gene that modulates MUC1 levels and promotes the initiation and progression of pancreatic intraepithelial neoplasia

doi: 10.1038/onc.2012.394

Figure Lengend Snippet: Pathological characterization of pancreatic disease in Pdx1-Cre/LSLKras G12D/+ /Smad4 lox/lox mice lacking none, one or both copies of Agr2

Article Snippet: Plasmids were obtained from: SBE4-Luc TGF-β reporter (Addgene #16495; [ ]), pCMV5-DPC-HA SMAD4 (Addgene #14038; [ ]), two pTRIPZ-AGR2 plasmids (Open Biosystems, Huntsville, AL; V2THS_251763, V2THS_199455), pcDNA-AGR2 and AGR2-RFP (Ted R. Hupp; University of Edinburgh).

Techniques:

Journal: Oncogene

Article Title: AGR2 is a SMAD4-suppressible gene that modulates MUC1 levels and promotes the initiation and progression of pancreatic intraepithelial neoplasia

doi: 10.1038/onc.2012.394

Figure Lengend Snippet: This figure shows serial sections of the same lesion, stained with either hematoxylin/eosin (column 1), co-IF for CK19 and amylase (column 2), Alcian blue (column 3), IF for AGR2 (column 4), or IF for MUC1 (column 5). (A) An mPanIN-1 lesion from Pdx1-Cre/Kras G12D /Smad4 L/L (AGR2 positiv e) . (B) An mPanIN-1 lesion from Pdx1-Cre/Kras G12D /Smad4 L/L /Agr2 −/− (AGR2 negative). (C) Low-grade adenocarcinoma from Pdx1-Cre/Kras G12D /Smad4 L/L /Agr2 +/− (AGR2 heterogeneous). (D) mPanIN-1/PanIN-2 lesion from Pdx1-Cre/Kras G12D /Smad4 L/L /Agr2 +/− (AGR2 heterogenous). Magnification: 100×; Scale bar: 40 μm.

Article Snippet: Plasmids were obtained from: SBE4-Luc TGF-β reporter (Addgene #16495; [ ]), pCMV5-DPC-HA SMAD4 (Addgene #14038; [ ]), two pTRIPZ-AGR2 plasmids (Open Biosystems, Huntsville, AL; V2THS_251763, V2THS_199455), pcDNA-AGR2 and AGR2-RFP (Ted R. Hupp; University of Edinburgh).

Techniques: Staining

Journal: Oncogene

Article Title: AGR2 is a SMAD4-suppressible gene that modulates MUC1 levels and promotes the initiation and progression of pancreatic intraepithelial neoplasia

doi: 10.1038/onc.2012.394

Figure Lengend Snippet: We describe a model in which, through one of potentially many mechanisms, AGR2 is negatively regulated by TGF-β signaling. In the presence of TGF-β, SMAD4 translocates to the nucleus, binds to SMAD-binding elements, and interacts with nuclear effectors of transcription. In the case of AGR2 , SMAD4 facilitates a repression of AGR2 transcription and prevents its downstream stabilization of MUC1 in the endoplasmic reticulum.

Article Snippet: Plasmids were obtained from: SBE4-Luc TGF-β reporter (Addgene #16495; [ ]), pCMV5-DPC-HA SMAD4 (Addgene #14038; [ ]), two pTRIPZ-AGR2 plasmids (Open Biosystems, Huntsville, AL; V2THS_251763, V2THS_199455), pcDNA-AGR2 and AGR2-RFP (Ted R. Hupp; University of Edinburgh).

Techniques: Binding Assay

Journal: European Journal of Dentistry

Article Title: Angiogenic Potential of Various Oral Cavity–Derived Mesenchymal Stem Cells and Cell-Derived Secretome: A Systematic Review and Meta-Analysis

doi: 10.1055/s-0043-1776315

Figure Lengend Snippet: Tabular representation of qualitative data obtained from literature search for included studies

Article Snippet: 3. , Yu et al , SCAPs , In vitro , HUVECs , VEGF and FGF-2 , RT-PCR and immunofluorescence staining , Both , No , BM-MSCs, dental pulp cells (DPCs) , SCAPs-CM showed enhanced osteogenic and neurogenic differentiation in DPCs but did not prove to be significant in angiogenesis.

Techniques: In Vivo, Real-time Polymerase Chain Reaction, Transfection, In Ovo, Membrane, Enzyme-linked Immunosorbent Assay, Negative Control, Positive Control, Derivative Assay, Activity Assay, Expressing, In Vitro, Inhibition, Immunofluorescence, Staining, Sequencing, Cell Surface Hydrophobicity, Flow Cytometry, Transmission Assay, Electron Microscopy, Migration, Concentration Assay, Microscopy, Over Expression, Western Blot, Fluorescence, Clinical Proteomics, Marker, Recombinant, Cell Culture, Produced, Plasmid Preparation, Cell Differentiation, Matrigel Assay, Control, Shear, Modification, Blocking Assay, Transduction, Ubiquitin Proteomics, Construct, Comparison

Journal: Drug Design, Development and Therapy

Article Title: Application of Topical Immunotherapy in the Treatment of Alopecia Areata: A Review and Update

doi: 10.2147/DDDT.S297858

Figure Lengend Snippet: Characteristics of Clinical Studies Using Squaric Acid Dibutylester for the Treatment of Alopecia Areata

Article Snippet: Kagami et al, 2020 , Case series , Refractory AA=4 , 2% DPCP or SADBE with 0.5% anthralin , 25% CR.

Techniques:

Journal: Drug Design, Development and Therapy

Article Title: Application of Topical Immunotherapy in the Treatment of Alopecia Areata: A Review and Update

doi: 10.2147/DDDT.S297858

Figure Lengend Snippet: Characteristics of Clinical Studies Using Diphenylcyclopropenone for the Treatment of Alopecia Areata

Article Snippet: Kagami et al, 2020 , Case series , Refractory AA=4 , 2% DPCP or SADBE with 0.5% anthralin , 25% CR.

Techniques:

Journal: Drug Design, Development and Therapy

Article Title: Application of Topical Immunotherapy in the Treatment of Alopecia Areata: A Review and Update

doi: 10.2147/DDDT.S297858

Figure Lengend Snippet: Characteristics of Clinical Studies Using Combination Therapy and Modified Protocol of Diphenylcyclopropenone for the Treatment of Alopecia Areata

Article Snippet: Kagami et al, 2020 , Case series , Refractory AA=4 , 2% DPCP or SADBE with 0.5% anthralin , 25% CR.

Techniques: Modification, Concentration Assay

Journal: Research

Article Title: Electrolyte Engineering for High-Voltage Lithium Metal Batteries

doi: 10.34133/2022/9837586

Figure Lengend Snippet: (a) Schematic illustration of the electrochemical deposition process of the Li metal anode. (b) Energy-based analysis (interfacial energy and strain energy) of Li dendrite formation. (c) The plot of the relationship between the interfacial energy for possible SEI components and the number of Li metal formula units. (d) Calculated interfacial energies γ , bulk modulus E from MP, and Li dendrite suppression ability γE for different interface components. DFT-optimized atomic structures of (e) LPS/Li and (f) LiF/Li interfaces and (g, h) their corresponding density of state (DOS) profiles by atomic layer with a Fermi level at 0 eV. The green, purple, yellow, and grey balls in (e, f) represent Li, P, S, and F atoms. (i) Thermodynamically stable interphase. (j) Reactive but forming an electron insulator SEI layer. (k) Reactive and forming a degradation layer with high electron conductivity. (m) Li potentials between the Li metal and the SSEs in the above three interphase types. (n) The difference between the green dash line (III) and the red dash (III′) is that the red dash line (III′) includes the overpotentials during the Li plating process. Reproduced with permission . Copyright 2018, American Association for the Advancement of Science. Illustrations of the solid full battery with (l) pristine LATP and (o) DPCE. (p) Cyclic voltammetry and (q) LSV curves of the DPCE at 60°C. (r) EIS analysis and (s) Arrhenius linear fitting plots of the DPCE at various temperatures. Reproduced with permission . Copyright 2019, American Chemical Society.

Article Snippet: To further explain the interfacial stability related to the ion manipulation triggered by the LATP ceramic in DPCE, COMSOL Multiphysics is conducted to explore the ion layer with high- t Li + .

Techniques: Battery